One of the Social Welfare Agency's covert squadron of schoolgirls-turned-trained-assassins, who protect Italy from terrorists, has disappeared. Now fellow hit girl Triela must go undercover to infiltrate a rival faction and bring her back alive.

The killer girls of Italy's Social Welfare Agency return in two featurettes that begin where the hit anime series "Gunslinger Girl" ended, with Rico and Jean on a mission to take out a Venice Mafia boss with terrorist connections. Later, Rico and Henrietta tag along with Jean and Jose when they head to their hometown for a respite -- but instead must come to grips with the emotional aftermath of their younger sister's death in a terrorist attack.